Product name
Ischemia-modified albumin (IMA), ELISA kit
Full Product Name
Human Ischemia Modified Albumin ELISA Kit
Product gene name: IMA ELISA kit
Species reactivity: Human
Specificity
This assay has high sensitivity and excellent specificity for the detection of AMI. No significant cross-reactivity or interference was observed between IMA and analogues. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the detection of cross-reactivity between IMA and all analogues, therefore cross-reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluids and tissue homogenate
Test type: Competitive or Sandwich
Sensitivity: 1.0 ug / mL
Preparation and Storage
Store all reagents at 2-8 degrees C
Sample preparation
We suggest prior experimentation with clean samples (undiluted), 1: 2 or 1: 4 dilutions. Avoid diluting your samples more than 1:10, as this would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team.
Product note
The information selected from the online data sheet is extracted from bioinformatics databases, which sometimes results in ambiguous or irrelevant information about the product. It is the customer’s responsibility to review, verify and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and can sometimes be updated and improved.
If the format of this assay is important to you, please request the current manual or contact our technical support team with a pre-sale consultation before placing an order. We will confirm the current details of the trial. We cannot guarantee that the sample manual posted online is the most up-to-date manual, it is intended to serve as an example only. See the instructions for use provided with the test kit for precise details.
Other notes
Small volumes of IMA ELISA kit vials can occasionally get caught in the product vial seal during shipping and storage. If necessary, briefly centrifuge the vial in a benchtop centrifuge to dislodge any liquid on the lid of the container. Certain products may require shipping with dry ice and an additional fee for dry ice may apply.
Searchable Terms for IMA Purchase
MBS728719 is a ready-to-use strip plate microwell ELISA (enzyme-linked immunosorbent assay) kit for testing the presence of target analytes of the Ischemia Modified Albumin (IMA) ELISA Kit in biological samples. The concentration gradients of the kit standards or positive controls generate a theoretical detection range of the kit in biological research samples containing IMA.
The biochemical analytical ELISA technique of the MBS728719 kit is based on IMA antibody-IMA antigen interactions (immunosorbent) and an HRP colourimetric detection system to detect IMA antigen targets in samples. The ELISA kit is designed to detect native, non-recombinant IMA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control trials that assessed reproducibility identified intra-assay CV (%) and inter-assay CV (%).
Product related information for ELISA ima kit
Intended Uses: This IMA ELISA kit is a 1.5-hour solid-phase ELISA designed for the quantitative determination of human IMA. This ELISA kit is for research only!
Assay principle: The IMA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been previously coated with a monoclonal antibody specific to IMA. Standards or samples are then added to the wells of the microtiter plate and, if present, the IMA will bind to the wells pre-coated with the antibody. To quantitatively determine the amount of IMA present in the sample, a standardized preparation of horseradish peroxidase-conjugated polyclonal antibody (HRP), specific for IMA, is added to each well to “sandwich” the immobilized IMA on the plate.
The microtiter plate is incubated and the wells are then washed thoroughly to remove all unbound components. Substrate solutions are then added to each well. The enzyme (HRP) and substrate are allowed to react for a short incubation period. Only wells containing IMA and enzyme-conjugated antibodies will show a colour change. The enzyme-substrate reaction is terminated by adding a sulfuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is drawn that relates the intensity of the colour (O.D.) to the concentration of the standards. The concentration of IMA in each sample is interpolated from this standard curve.
Cautions
All MyBioSource products are for scientific laboratory research purposes and not for diagnostic, therapeutic, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Product purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order when we reasonably believe that the intended use will not conform to our acceptable guidelines.
Disclaimer
While every effort was made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be responsible for any omissions or errors contained in this document. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
