Exploring the Demographics and Scientific Traits Associated to the Expression of Angiotensin-Changing Enzyme 2, a Receptor of SARS-CoV-2
Goal: Coronavirus illness 2019 (COVID-19) was first reported in Wuhan, China, and has quickly unfold all through the world. It has been reported that angiotensin-converting enzyme 2 (ACE2) is likely one of the main mobile entry receptors of SARS-CoV-2; thus, excessive ACE2 expression could enhance susceptibility to an infection. Subsequently, we analyzed the expression of ACE2 within the blood to determine the people who could also be inclined to an infection.
Strategies: In complete, 229 topics had been enrolled on this examine, and reverse transcription-quantitative polymerase chain response and ELISA assay was used to determine the extent of ACE2 mRNA expression and ACE2 protein stage within the blood. Demographic and scientific traits, together with age, gender, weight, top, smoking habits, ingesting habits, diabetes, and hypertension, had been obtained utilizing a face-to-face questionnaire. Impartial Scholar’s t-test, Pearson’s linear correlation, logistic regression evaluation, and a number of linear regression correlation had been carried out to evaluate the affiliation between these components and the expression of ACE2.
Outcomes: Increased stage of ACE2 was noticed in females, older topics, topics with hypertension, topics with a cardiocerebrovascular illness, male people who smoke, and topics with most cancers (p < 0.05) than in different topics. A number of linear regression evaluation confirmed that there’s a statistically vital correlation between being a feminine and ACE2 expression (β = 0.550, p < 0.001), between older age and ACE2 expression (β = 0.197, p = 0.003), between smoking and ACE2 expression (β = 0.163, p = 0.037),
and between most cancers and ACE2 expression (β = 0.265, p < 0.001). Logistic regression evaluation revealed that feminine topics (odds ratio [OR] = 2.255, 95% confidence interval [CI] = 1.770-2.872), topics with hypertension (OR = 1.264, 95% CI = 1.075-1.486), topics with a cardiocerebrovascular illness (OR = 1.271, 95% CI = 1.023-1.579), topics with most cancers (OR = 1.695, 95% CI = 1.253-2.293), and topics above 60 years of age (OR = 3.097, 95% CI = 1.078-8.896) are at an elevated threat of an infection on account of their excessive expression of ACE2.
Conclusion: The extent of ACE2 is increased in females, older topics, people who smoke, and topics with most cancers than in different topics, indicating that a few of that are at increased threat for the extreme types of COVID-19 when they’re uncovered to the SARS-Cov-2.
Description: A competitive ELISA for quantitative measurement of Rat Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativesandwich ELISA kit for measuring Pig creatine kinase MB isoenzyme (CK-MB) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Pig creatine kinase MB isoenzyme (CK-MB) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Rat Creatine Kinase MB isoenzyme, CK-MB in samples from serum, urine, tissue homogenates, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rat Creatine Kinase MB isoenzyme, CK-MB in samples from serum, urine, tissue homogenates, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive ELISA for quantitative measurement of Human Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Creatine Kinase MB isoenzyme in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativesandwich ELISA kit for measuring Human Creatine Kinase MB isoenzyme, CK-MB in samples from serum, plasma, urine, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Human Creatine Kinase MB isoenzyme, CK-MB ELISA Kit
Description: Quantitativesandwich ELISA kit for measuring Human Creatine Kinase MB isoenzyme, CK-MB in samples from serum, plasma, urine, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Mouse Creatine Kinase MB isoenzyme, CK-MB in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse Creatine Kinase MB isoenzyme, CK-MB in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Human Creatine Kinase MB isoenzyme(CK-MB)ELISA Kit
Description: Quantitativesandwich ELISA kit for measuring Rabbit creatine kinase MB isoenzyme (CK-MB) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rabbit creatine kinase MB isoenzyme (CK-MB) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativecompetitive ELISA kit for measuring Canine Creatine Kinase MB isoenzyme (CK-MB) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativecompetitive ELISA kit for measuring Canine Creatine Kinase MB isoenzyme (CK-MB) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich CLIA kit for quantitative measurement of Rat CK-MB (Creatine Kinase MB Isoenzyme) in samples from Serum, Plasma, Cell supernatant
Description: Quantitative sandwich ELISA for measuring Rat Creatine Kinase MB isoenzyme (CK-MB) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Rat Creatine Kinase MB isoenzyme (CK-MB)
Description: Quantitative sandwich ELISA for measuring Rat Creatine Kinase MB isoenzyme (CK-MB) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Rat Creatine Kinase MB isoenzyme (CK-MB)
Description: Quantitative sandwich ELISA for measuring Rat Creatine Kinase MB isoenzyme (CK-MB) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Creatine Kinase MB Isoenzyme (CKMB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Creatine Kinase MB Isoenzyme (CKMB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Creatine Kinase MB Isoenzyme (CKMB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Creatine Kinase MB Isoenzyme (CKMB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Creatine Kinase MB Isoenzyme (CKMB) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CKMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CKMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CKMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CKMB in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CKMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CKMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CKMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CKMB in the samples is then determined by comparing the OD of the samples to the standard curve.
Enzyme-Impressed Meeting: Incorporating Multivariate Interactions to Optimize the Host-Visitor Configuration for Excessive-Velocity Enantioselective Catalysis
To realize a speedy asymmetry conversion, the substrate objects undergo from the accelerated kinetic velocity and ran-dom rotation at the price of selectivity. Impressed by pure enzyme, optimizing the host-guest configuration will real-ize the high-performance enantioselective conversion of chemical reactions.
Herein, multivariate binding interac-tions had been launched into the 1D channel of a chiral catalyst to simulate the enzymatic motion. An imidazolium group was used to electrophilically activate the C=O unit of a ketone substrate and the counterion bounds the hy-drogen-donor isopropanol.
This binding impact across the catalytic heart produces robust stereo-induction, result-ing in excessive conversion (99.5% yield) and enantioselectivity (99.5% ee) for the uneven hydrogenation of bio-mass-derived acetophenone.
As well as, the turnover frequency of the ensuing catalyst (5160 h-1 TOF) is over 58 occasions that of a homogeneous Ru-TsDPEN catalyst (88 h-1 TOF) below the identical situation, which corresponds to the most effective efficiency reported thus far amongst all present catalysts for the thought of response.
Antidiabetic and In VitroEnzyme Inhibition Analysis of Methanol Extract of Ocimumtenuiflorum Linn Leaves and Its Fractions
The current analysis aimed to seek out out the easiest dose of methanol extract of Ocimumtenuiflorum leaves extract, and it is a fraction to blood-glucose-reducing in diabetic rats, and evaluated the α-amylase, α-glucosidase inhibitors and insulin diploma of diabetic rats used to achieve bigger administration over hyperglycemia.
The outcomes of the antihyperglycaemic of oral administration of a particular dose of methanol extract in streptozotocin-induced rats confirmed that the most effective dose of methanol extract significantly diminished the blood glucose diploma compared with one different dose.
Moreover, the outcomes of repeated administration of methanol fractions signifies that ethyl acetate-butanol fraction exhibited a stronger antihyperglycemic affect than chloroform and ethanol-water fractions. Moreover, the end result confirmed that affect of methanol extract and its fraction on α-glucosidase and α-amylase enzymes actions and its insulin diploma by in vitro analysis, ethyl acetate-butanol fraction could administration with low focus compared with completely different fractions and acarbose that used as a constructive administration.
From the outcomes of insulin diploma, methanol extract and fraction did not current any necessary. These findings indicated that the energetic crude extract (methanol) and its energetic fractions (ethyl acetate/butanol) could exert necessary glucose-lowering affect due to the presence of polyphenolics energetic constituents. In conclusion, isolation of the energetic components of Ocimumtenuiflorum might pave the best way wherein to the occasion of newest brokers for the remedy of diabetes and its points.
Description: This product includes 500 ul of 10 x Buffer DP, 35 ul of 100 x DNA template, 35 ul of 100x dNTP mix and 320 ul of 10x fluorescence dye for 100 assays of DNA polymerase reactions in a 384-well assay format. The assay conditions are optimized for bacterial DNA polymerase III alpha subunit. Enzyme is not included in the kit.
Description: This product includes 400 ul of 10 x Buffer, 33 ul of 100 x DNA, 33 ul of 100 x NTPs, 1550 ul of 2 x Dye and 1550 ul of 50 mM EDTA. It is for 100 assays of T7 RNA polymerase. The assay This product includes all reagents except the enzyme.
T7 RNA Polymerase Assay Kit Plus (enzyme included)
Description: This product includes all reagents in T7 RNA Polymerase Assay Kit (Catalog number T7RPA100K) plus the enzyme, 33 ul of 100 x T7 RNA polymerase.
Description: This product includes the reaction buffer (T1 Buffer), supercoiled plasmid DNA and fluorescence dye H19 for assaying 20 DNA relaxation reactions in a 96-well plate assay format or 40 assays in a 384-well assay format. The reaction buffer is optimized for bacterial topoisomerase I.
E. coli DNA Primase Assay Kit (enzyme not included)
Description: This product includes 600 ul of 10 x assay buffer, 45 ul of 100 x DNA template, 45 ul of 100 x NTP mix and 850 ul of 10 x fluorescence dye for 100 assays of E. coli DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format. The kit does not include the enzyme.
Description: This product includes 600 ul of 10 x assay buffer, 45 ul of 100 x DNA template, 45 ul of 100 x NTP mix and 850 ul of 10 x fluorescence dye for 100 assays of S. aureus DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format. The kit does not include the enzyme.
AMV Reverse Transcriptase Assay Kit (enzyme not included)
Description: This product includes 400 ul of 10 x Buffer, 33 ul of 100 x Template, 33 ul of 100 x dNTPs, 1550 ul of 2 x Dye and 1550 ul of 50 mM EDTA. It is for 100 assays of AMV Reverse Transcriptase. The assay This product includes all reagents except the enzyme.
HIV Reverse Transcriptase Assay Kit (enzyme not included)
Description: This product includes 400 ul of 10 x Buffer, 33 ul of 100 x Template, 33 ul of 100 x dNTPs, 1550 ul of 2 x Dye and 1550 ul of 50 mM EDTA. It is for 100 assays of HIV Reverse Transcriptase. The assay This product includes all reagents except the enzyme.
Insulin Degrading Enzyme (IDE) Magnetic Luminex Assay Kit
Description: This product includes all the kit components in S. aureus DNA Primase Assay Kit (Catalog No. AGA100K ) plus 45 ul of 100 x S. aureus primase. It is for 100 assays of S. aureus DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format.
E. coli RNA polymerase Assay Kit (enzyme not included)
Description: This product includes 400 ul of 10 x Buffer, 33 ul 100 x DNA, 33 ul of 100x NTPs, 350 ul of 10 x fluorescence dye. The assay This product includes all reagents except the enzyme. It is for 100 assays of E. coli RNA polymerase reactions in a 384-well assay format.
E. coli gyrase ATPase assay Kit Plus (enzyme included)
Description: This product includes the reaction buffer, DNA, E. coli gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.
AMV Reverse Transcriptase Assay Kit Plus (enzyme included)
Description: This product includes all reagents in the AMV Reverse Transcriptase Assay Kit (Catalog number: AMV100K) plus the enzyme, 7 ul of 500 x AMV RT.
HIV Reverse Transcriptase Assay Kit Plus (enzyme included)
Description: This product includes all reagents in the HIV Reverse Transcriptase Assay Kit (Catalog number: HIV100K) plus the enzyme, 7 ul of 500 x HIV RT.
Glycogen Debranching Enzyme (AGL) Magnetic Luminex Assay Kit
Description: This product includes 600 ul of 10 x assay buffer, 45 ul of 100 x DNA template, 45 ul of 100 x NTP mix and 850 ul of 10 x fluorescence dye for 100 assays of S. pneumoniae DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format. The kit does not include the enzyme.
S. aureus RNA polymerase assay kit (enzyme not included)
Description: This product includes 400 ul 10 x Buffer, 33 ul 100 x DNA, 33 ul 100x NTPs, 350 ul 10 x fluorescence dye. The assay This product includes all reagents except the enzyme. It is for 100 assays of S. aureus RNA polymerase reactions in a 384-well assay format.
S. aureus gyrase ATPase assay Kit Plus (enzyme included)
Description: This product includes the reaction buffer, DNA, S. aureus gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.
Human DNA polymerase beta assay kit (enzyme not included)
Description: This product includes all the assay reagents except the enzyme for 100 assays of human DNA polymerase beta reactions in a 96-well plate format.
S. pneumonia DNA Primase Assay Kit Plus (enzyme included)
Description: This product includes all the kit components in S. pneumoniae DNA Primase Assay Kit (Catalog No. PGA100K ) plus 45 ul of 100 x S. pneumoniae primase. It is for 100 assays of S. pneumoniae DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format.
H. influenzae DNA Primase Assay Kit (enzyme not included)
Description: This product includes 600 ul of 10 x assay buffer, 45 ul of 100 x DNA template, 45 ul of 100 x NTP mix and 850 ul of 10 x fluorescence dye for 100 assays of H. influenzae DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format. The kit does not include the enzyme.
S. aureus RNA polymerase assay kit Plus (enzyme included)
Description: This product includes all the reagents in S. aureus RNA Polymerase Assay Kit (Catalog number RPA100KS) plus the enzyme, 33 ul 100 x S. aureus RNAP. It is for 100 assays of S. aureus RNA polymerase reactions in a 384-well assay format.
NAD-dependent DNA Ligase Assay Kit (enzyme not included)
Description: This product includes 800 ul of 10 x buffer, 44 ul of 100x DNA substrate, 44 ul of 100 x NAD+, 21 ml of Reagent T and 220 ul of 10 x fluorescence dye for 100 assays. The kit does not include ligase that can be purchased separately (please see the information of related products).
H. influenzae DNA Primase Assay Kit Plus (enzyme included)
Description: This product includes all the kit components in H. influenzae DNA Primase Assay Kit (Catalog No. HGA100K ) plus 45 ul of 100 x H. influenzae primase-helicase complex. It is for 100 assays of H. influenzae DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format.
E. coli Topo IV ATPase Assay Kit Plus (enzyme included)
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x E. coli Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of E. coli Topo IV ATPase activity in a 384-well assay format.
Description: This product includes the reaction buffer (T2 Buffer), relaxed plasmid DNA and fluorescence dye H19 for 20 assays of DNA supercoiling reactions a 96-well plate assay format or 40 assays in a 384-well assay format.
E. coli Thymidylate Kinase Assay Kit Plus (enzyme included)
Description: This product includes 600 ul of 10 xi Buffer, 35 ul of 100 x dTMP, 35 ul of 100 x E. coli Thymidylate kinase, 35 ul of 100 x ATP, 35 ul of 100 x MUK Reagent A, 35 ul of 100 x MUK Reagent B and 300 ul of 10 x fluorescence dye for 100 assays in a 384-well assay format.
Description: This product includes the reaction buffer, DNA, P. aeruginosa gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.
Description: This product includes all the reagents in the DNA Polymerase III Alpha Assay Kit (Catalog No. DPA100K) plus 35 ul of 100 x H. influenzae DNA polymerase III alpha subunit.
S. pneumoniae DNA Polymerase Assay Kit Plus (enzyme included)
Description: This product includes all the reagents in the DNA Polymerase III Alpha Assay Kit (Catalog No. DPA100K) plus 35 ul of 100 x S. pneumoniae DNA polymerase III alpha subunit.
M-MLV Reverse Transcriptase Assay Kit (enzyme not included)
Description: This product includes 400 ul of 10 x Buffer, 33 ul of 100 x Template, 33 ul of 100 x dNTPs, 1550 ul of 2 x Dye and 1550 ul of 50 mM EDTA. It is for 100 assays of M-MLV Reverse Transcriptase. The assay This product includes all reagents except the enzyme.
Human Thymidylate Kinase Assay Kit Plus-500 (enzyme included)
Description: This product includes 1700 ul of 10 x reaction buffer, 8 ul of 2500 x human thymidylate kinase (125 uM), 170 ul of 100 x ATP (5 mM), 170 ul of 100 x dTMP (40 mM), 170 ul of 100 x MUK Reagent A, 170 ul of 100 x MUK Reagent B and 1700 ul of 10 x fluorescence dye. The kit reagents are sufficient for 500 thymidylate kinase assays using a standard black 384-well plate (Matrix 4318).
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x S. pneumoniae Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of S. pneumoniae Topo IV ATPase activity in a 384-well assay format.
Angiotensin I Converting Enzyme (ACE) Magnetic Luminex Assay Kit
Description: This product includes 150 ul of 10 x assay buffer (Buffer T4), 105 ul of 10 x concatenated DNA, 30 ul of 50 x ATP (10 mM),120 ul of 0.4 M EDTA, 160 ul of 20 x fluorescence dye and 20 spin columns for 20 DNA decatenation assays. The assay buffer is optimized for bacterial topoisomerase IV. Bacterial topoisomerase IV enzymes are provided separately.
Description: CheKine™ Micro Starch Branching Enzyme(SBE) Activity Assay Kitis designed for Starch Branching Enzyme(SBE) Activity detecting in the sample.
Description: CheKine™ Micro Starch Branching Enzyme(SBE) Activity Assay Kitis designed for Starch Branching Enzyme(SBE) Activity detecting in the sample.
E. coli Thymidylate Kinase Assay Kit Plus-500 (enzyme included)
Description: This product includes the 10 x reaction buffer (10 x MUK buffer), E. coli thymidylate Kinase, dTMK substrate, 100 x MUK reagent 1, 100 x MUK reagent 2 and 10 x MUK dye. The kit reagents are sufficient for 500 assays of E. coli thymidylate kinase using a standard black or white 384-well plate.