Murine RNase Inhibitor

The murine RNase inhibitor is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding non-covalently in a 1: 1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H, or RNase from Aspergillus. Furthermore, no inhibition of polymerase activity is observed when the RNase inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7 or T3).

Description

The murine RNase inhibitor is a 50 kD recombinant protein isolated from E. coli that contains the mouse native ribonuclease inhibitor gene. Our RNase inhibitor product has specificity for RNases A, B, and C, but was unable to inhibit RNase 1, RNase T1, nuclease S1, RNase H, or Aspergillus RNase. It can bind to various types of RNase non-covalently in a 1: 1 ratio with high affinity.

Especially, according to previous research, no inhibition of enzymatic activity is observed when RNase inhibitor is used within AMV or M-MuLV reverse transcriptases, Taq DNA polymerase or SP6 / T7 / T3 RNA polymerases, which means that it can be taken in various experiments to prevent RNA degradation.

Compared to human RNase, the mouse RNase inhibitor lacks the cysteine ​​pair that is very sensitive to oxidation and leads to the inactivation of the inhibitor. As a consequence, RNase Inhibitor, Murine significantly improves its resistance to oxidation compared to RNase inhibitors of human/porcine origin.

It is stable at low concentrations of DTT (less than 1 mM), and this character makes it preferred for reactions in which a low concentration of DTT is required for the reaction (eg, real-time RT-PCR). Our product can be used in many types of reactions to prevent RNA degradation, for example, RT-PCR, cDNA synthesis, in vitro transcription, enzymatic RNA labelling reaction or any other application.

Characteristics

  • The product specifically inhibits RNases A, B and C.
  • Improved resistance to oxidation compared to human / porcine RNase inhibitor
  • Ideal for reactions where a low concentration of DTT is required (eg, real-time PCR)

Storage

Store components at -20 ° C.