Cell Cycle Assay Kit (Fluorometric – Green)
Detection method: Fluorescent
Example type: Adherent cells, Cells in suspension
Reacts with: Mammals, Other species
The Cell Cycle Assay Kit (Fluorometric – Green) (ab112116) is designed to monitor cell cycle progression and proliferation using our proprietary Nuclear Green CCS1 in living cells. The percentage of cells in a given sample that is in the G0 / G1, S, and G2 / M phases, as well as cells in the sub-G1 phase prior to apoptosis, can be determined by flow cytometry. Nuclear Green CCS1 stained cells can be monitored with a flow cytometer at Ex / Em = 490 nm / 520 nm (channel FL1).
Live Cell Cycle Analysis Stains: Vybrant DyeCycle Stains
We offer classic DNA cell cycle stains such as Hoechst 33342 and DRAQ5 for cell cycle analysis, but most of these have limitations that must be considered when using them in an experiment which is why the Invitrogen Vybrant DyeCycle stains were developed for live cell cycle analysis.
Vybrant DyeCycle dyes are:
- Accurate: precise analysis of the cell cycle in living cells
- Safe – low cytotoxicity to combine with additional live cell experiments
- Cell Sort Support – Easily sort cells based on the cell cycle phase
- Flexible: dyes available for all common laser lines (UV, 405, 488, 532 and 633 nm)
Easily combine DyeCycle stains with other live-cell applications
Combining cell cycle analysis with additional live cell applications (i.e., GFP cell analysis, immunophenotyping, cell sorting, CFSE cell screening, and sub-G1 apoptotic population analysis) is simple with Vybrant DyeCycle Stains. These stains are cell-permeable nucleic acid stains that can penetrate the nucleus without cellular fixation.
If Vybrant DyeCycle stains are used in combination with other stains for multi-colour applications, apply the other stains to the sample first, following all manufacturer’s instructions, including washing steps. The dye cycle stain should be the last stain applied to the sample and do not wash or fix the samples prior to flow cytometry analysis.
Identify Secondary Stem Cell Populations Using DyeCycle Violet
Vybrant DyeCycle Violet Stain has been shown to identify secondary populations (CD34– cells) in stem cells and thus allow a phenotype by which to isolate this type of cell population. The basis of the secondary population technique is that human and rodent stem cells expel the Vybrant DyeCycle Violet stain (and also the Hoechst 33342 stain). The efflux can be blocked with verapamil, fumitermorgin C, or other similar blocking agents, to prevent the efflux of dye for accurate analysis of DNA content in these stem cells.